全文获取类型
收费全文 | 318939篇 |
免费 | 17685篇 |
国内免费 | 659篇 |
专业分类
林业 | 24383篇 |
农学 | 14214篇 |
基础科学 | 2995篇 |
45459篇 | |
综合类 | 45288篇 |
农作物 | 17062篇 |
水产渔业 | 18113篇 |
畜牧兽医 | 137543篇 |
园艺 | 6548篇 |
植物保护 | 25678篇 |
出版年
2019年 | 3743篇 |
2018年 | 4345篇 |
2017年 | 4778篇 |
2016年 | 5097篇 |
2015年 | 4502篇 |
2014年 | 5884篇 |
2013年 | 16593篇 |
2012年 | 8108篇 |
2011年 | 10451篇 |
2010年 | 8007篇 |
2009年 | 8423篇 |
2008年 | 9715篇 |
2007年 | 8927篇 |
2006年 | 9375篇 |
2005年 | 8569篇 |
2004年 | 8397篇 |
2003年 | 7992篇 |
2002年 | 7376篇 |
2001年 | 8497篇 |
2000年 | 8002篇 |
1999年 | 6976篇 |
1998年 | 4119篇 |
1997年 | 4156篇 |
1996年 | 4011篇 |
1995年 | 4821篇 |
1994年 | 4266篇 |
1993年 | 3974篇 |
1992年 | 5947篇 |
1991年 | 6200篇 |
1990年 | 5960篇 |
1989年 | 6023篇 |
1988年 | 5450篇 |
1987年 | 5563篇 |
1986年 | 5632篇 |
1985年 | 5665篇 |
1984年 | 5031篇 |
1983年 | 4640篇 |
1982年 | 3597篇 |
1981年 | 3410篇 |
1980年 | 3328篇 |
1979年 | 4394篇 |
1978年 | 3824篇 |
1977年 | 3464篇 |
1976年 | 3159篇 |
1975年 | 3111篇 |
1974年 | 3726篇 |
1973年 | 3766篇 |
1972年 | 3543篇 |
1971年 | 3345篇 |
1970年 | 3210篇 |
排序方式: 共有10000条查询结果,搜索用时 203 毫秒
81.
Prevalence,geographic distribution and phenotypic differences of Piscirickettsia salmonis EM‐90‐like isolates 下载免费PDF全文
J Saavedra N Hernandez A Osses A Castillo A Cancino H Grothusen E Navas P Henriquez H Bohle F Bustamante P Bustos M Mancilla 《Journal of fish diseases》2017,40(8):1055-1063
Early reports accounted for two main genotypes of Piscirickettsia salmonis, a fish pathogen and causative agent of piscirickettsiosis, placing the single isolate EM‐90 apart from the prototypic LF‐89 and related isolates. In this study, we provide evidence that, contrary to what has been supposed, the EM‐90‐like isolates are highly prevalent and disseminated across Chilean marine farms. Molecular analysis of 507 P. salmonis field isolates derived from main rearing areas, diverse hosts and collected over 6 years, revealed that nearly 50% of the entire collection were indeed typed as EM‐90‐like. Interestingly, these isolates showed a marked host preference, being recovered exclusively from Atlantic salmon (Salmo salar) samples. Although both strains produce undistinguishable pathological outcomes, differences regarding growth kinetics and susceptibility to the antibiotics and bactericidal action of serum could be identified. In sum, our results allow to conclude that the EM‐90‐like isolates represent an epidemiologically relevant group in the current situation of piscirickettsiosis. Based on the consistency between genotype and phenotype exhibited by this strain, we point out the need for genotypic studies that may be as important for the Chilean salmon industry as the continuous surveillance of antimicrobial susceptibility patterns. 相似文献
82.
Body shape variation in two species of darters (Etheostoma,Percidae) and its relation to the environment 下载免费PDF全文
Environmental variation can shape phenotypic variation in organisms, but most evidence for trait differentiation comes from analyses of dichotomous habitat types that differ in only one or few key environmental factors. In reality, environmental variation is often more subtle, gradual and multifarious. Here, we investigated geographic variation in body shape of two darter species (Etheostoma spectabile and Etheostoma flabellare; Percidae) that occur along river gradients. This study addressed three specific questions: Is there intraspecific geographic variation in the two species across different sites in the Ozark Highlands of Oklahoma (USA)? Is phenotypic variation across sites correlated with abiotic environmental conditions? Do the two species share site‐specific (i.e. convergent) phenotypic variation in areas where they occur together? Our results indicated significant body shape variation in both species. Population differences in body shape were particularly correlated with variation in substrate composition. The combined analysis of both species indicated a small but significant effect of convergence on body shape wherever they are sympatric; shared variation, however, was not related to any environmental variables included in the analysis. While it remains unclear whether phenotypic variation in these species is due to heritable differentiation or environmentally induced plasticity, our results indicate that even subtle and gradual environmental variation can induce substantial variation in phenotypes on a relatively small spatial scale. 相似文献
83.
R. C. Silva S. Liebel H. H. P. de Oliveira W. A. Ramsdorf J. R. E. Garcia S. M. F. O. Azevedo V. F. Magalhães C. A. Oliveira Ribeiro F. Filipak Neto 《Fish physiology and biochemistry》2017,43(5):1237-1244
Cylindrospermopsin (CYN) is a cyanotoxin that is cytotoxic to a wide variety of cells, particularly to the hepatocytes. In this study, the toxic effects of purified CYN were investigated in primary cultured hepatocytes of Neotropical fish Hoplias malabaricus. After isolation, attachment, and recovery for 72 h, the cells were exposed for 72 h to 0, 0.1, 1.0, 10, and 100 μg l?1 of CYN. Then, cell viability and a set of oxidative stress biomarker responses were determined. Catalase, superoxide dismutase, glucose-6-phosphate dehydrogenase, and glutathione S-transferase activities were not affected by exposure to CYN. Concentration-dependent decrease of glutathione reductase activity occurred for most CYN-exposed groups, whereas non-protein thiol content increased only for the highest CYN concentration. Lipid peroxidation, protein carbonylation, and DNA damage levels were not altered, but reactive oxygen species levels increased in the cells exposed to the highest concentration of CYN. Cell viability decreased in all the groups exposed to CYN. Thus, CYN may cause a slight change in redox balance, but it is not the main cause of cell death in H. malabaricus hepatocytes. 相似文献
84.
M. Torfi Mozanzadeh V. Yavari J.G. Marammazi N. Agh E. Gisbert 《Aquaculture Nutrition》2017,23(3):470-483
Four isonitrogenous diets containing different carbohydrate:lipid (CHO:L) ratios (0.3, 0.6, 1.1 and 1.8) were tested in triplicate groups (16 fish per replicate) of silvery‐black porgy juveniles for 8 weeks. Growth performance was not affected by different dietary CHO:L ratios (P > 0.05); however, the viscerosomatic index, the intraperitoneal fat, whole‐body lipid, energy and n‐3 long chain polyunsaturated fatty acids levels increased with decreasing dietary CHO:L ratios (P < 0.05). Fish fed with 1.8 CHO:L diet had the lowest apparent digestibility coefficients of protein and lipid, as well as the lowest plasma haemolytic and lysozyme activities (P < 0.05). Red blood cell counts and plasma glucose levels were higher in fish fed with 1.1 and 1.8 CHO:L ratio diets than in the other groups (P < 0.05). Plasma alkaline phosphatase and alanine aminotransferase, as well as the level of lipid peroxidation and total antioxidant capacity in the liver and plasma increased as dietary CHO:L ratios decreased (P < 0.05). The results of the current study indicated that the diets with CHO:L ratios between 0.6 and 1.1 are optimal for silvery‐black porgy, whereas higher ratios may result in hyperglycaemia and immune suppression, and lower CHO:L ratios may lead to oxidative stress and liver dysfunction. 相似文献
85.
86.
M. Espe S.M. Andersen E. Veiset‐Kent I. Rønnestad E. Holen J.‐E. Zerrahn A. Aksnes 《Aquaculture Nutrition》2017,23(5):1086-1094
Methionine–choline‐deficient (MCD) mammals are known to accumulate liver TAG probably due to phosphatidylcholine (PC) deficiency and thus assembly of VLDL and transport of lipids from liver to peripheral organs. To assess whether supplementation of choline could spare methionine and secure a healthy liver metabolism, by reducing the endogenous PC synthesis without interfering with lipid transport and distribution, Atlantic salmon with initial BW of 700 g were fed adequate (1.9 g Met/16 gN) or surplus methionine (2.5 g Met/16 gN) diets of which were supplemented with choline or not for a period of 19 weeks. Fish fed the low‐methionine diets had reduced growth (p = .013) due to reduced protein gain (p = .007), while lipid gain slightly improved in fish fed the choline‐supplemented diets (p = .047). Also, feed conversion improved when fed surplus methionine (p < .001), while choline supplementation had no impact on feed conversion. No interaction between choline and methionine on growth performance or retention existed. Phospholipid status in liver and muscle was not affected by treatments, and no liver TAG accumulation occurred at the methionine levels used. Gene expression of ApoB100 necessary for assembling VLDL or pemt necessary for endogenous PC synthesis was unaffected by treatments. Capacity of methylation (MAT, BHMT) within the liver was not affected by treatment nor was the gene expression of enzymes in liver transsulfuration (CBS or CDO). Methionine status within liver was unaffected by treatments, while free methionine reduced in those fish fed the low‐methionine diets in muscle and plasma. Cystathionine and taurine were elevated when fed surplus methionine. Choline supplementation had no impact on sulphur amino acid metabolites in either tissue. Neither did choline supplementation improve TAG mobilization from liver to muscle. To conclude, choline does not improve endogenous phospholipid synthesis or transport of TAG from liver to muscle depot when added to diets containing 1.9 g Met/16 gN, while surplus methionine improved growth and protein retention, indicating that 1.9 g Met/16 gN is enough to support a healthy liver metabolism, but too low to support muscle protein deposition in adult salmon fed high plant protein diets for longer periods of time. 相似文献
87.
Isolation of infectious microalga Prototheca wickerhamii from a carp (Cyprinus carpio) – a first confirmed case report of protothecosis in a fish 下载免费PDF全文
Protothecosis is a rare infection caused by environmentally ubiquitous achlorophyllic microalgae of the genus Prototheca. Here, we describe a first case of protothecosis in a carp (Cyprinus carpio), which is at the same time the first case of protothecosis in a fish, confirmed by phenotype‐ and molecular‐based methods, including PCR sequencing of the rDNA cluster and protein profiling using matrix‐assisted laser desorption ionization time‐of‐flight mass spectrometry. 相似文献
88.
Effect of dietary protein content on growth rate,survival and body composition of juvenile cauque river prawn,Macrobrachium americanum (Bate 1868) 下载免费PDF全文
Yuniel Méndez‐Martínez Stig Yamasaki‐Granados Marcelo U García‐Guerrero Luis R Martínez‐Córdova Marta E Rivas‐Vega Fabiola G Arcos‐Ortega Edilmar Cortés‐Jacinto 《Aquaculture Research》2017,48(3):741-751
We determined the effect of four concentrations of dietary crude protein, 30.7, 37.2, 41.8 and 46.8% on growth rate, survival and body composition of the juvenile cauque river prawn (Macrobrachium americanum). The prawns were hatched in the laboratory from the spawn of one wild ovigerous female. Prawns consuming 37.2% crude protein reached a final weight of 0.58 g (feed conversion ratio of 2.15), which was significantly better than the other treatments. Survival was 100% in all treatments. Protein content in the diets had no significant effect on whole body proximate composition and amino acid profile. Juveniles consuming the 37.2% crude protein diet grew faster than those fed the other diets. Specific growth rate was adjusted to the two‐slope broken‐line regression analysis model to estimate the optimal protein requirement. In conclusion, these results demonstrated that the 37.2% protein level diet is optimal for juvenile cauque river prawn M. americanum in the experimental conditions of this study. 相似文献
89.
Immunostimulatory effects of dietary poly‐β‐hydroxybutyrate in European sea bass postlarvae 下载免费PDF全文
Andrea Franke Catriona Clemmesen Peter De Schryver Linsey Garcia‐Gonzalez Joanna J Miest Olivia Roth 《Aquaculture Research》2017,48(12):5707-5717
The stable production of high‐quality fry in marine aquaculture is still hampered by unpredictable mortality caused by infectious diseases during larval rearing. Consequently, the development of new biocontrol agents is crucial for a viable aquaculture industry. The bacterial energy storage compound poly‐β‐hydroxybutyrate (PHB) has been shown to exhibit beneficial properties on aquatic organisms such as enhanced survival, growth, disease resistance and a controlling effect on the gastrointestinal microbiota. However, the effect of PHB on the developing immune system of fish larvae has so far not been investigated. In this study, the effect of feeding PHB‐enriched Artemia nauplii on survival, growth and immune response in European sea bass (Dicentrarchus labrax) postlarvae was examined. Amorphous PHB was administered to 28‐day‐old sea bass postlarvae over a period of 10 days. The survival and growth performance were monitored, and the expression of 29 genes involved in immunity, growth, metabolism and stress‐response was measured. While the expression of the insulin‐like growth factor 1 (igf1), an indicator of relative growth, was upregulated in response to feeding PHB, the larval survival and growth performance remained unaffected. After 10 days of PHB treatment, the expression of the antimicrobial peptides dicentracin (dic) and hepcidin (hep) as well as mhc class IIa and mhc class IIb was elevated in the PHB fed postlarvae. This indicates that PHB is capable of stimulating the immune system of fish early life stages, which may be the cause of the increased resistance to diseases and robustness observed in previous studies. 相似文献
90.
Richard J Beamish 《Fish and Fisheries》2017,18(6):1161-1175
The Larkin lectures are held every two years at the University of British Columbia in recognition of Dr. Peter Larkin's contributions to fisheries science. The lecture I presented in November 2015 coincided with an announcement that the Institute of Fisheries that Peter Larkin founded in the 1960s would be restructured as the “Institute for Oceans and Fisheries” with an emphasis on fisheries and oceans issues important to British Columbia as well as the rest of the world. I decided to look back at research issues that Peter Larkin thought would be important for Pacific salmon (Oncorhynchus spp.) in the future and see what has happened as a way of identifying the complexity that the Institute for Oceans and Fisheries in particular and the science community in general will face. I chose five themes from Peter Larkin's talks: 1 understanding marine survival, 2 ocean carrying capacity, 3 aquaculture, 4 climate, Pacific salmon and climate change and 5 informing the public, and then added my opinion about research priorities for the future. Peter Larkin recognized the future relevance of these examples, but he probably could not have imagined how these and related issues will challenge his renamed institute and the rest of the research community over the next 50 years. 相似文献